| Project: | Mouse Fasting Liver and Hepatocyte w/ FSK |
| Species: | Mouse |
| Date: | 2013-05-21 |
| URL: | PNAS 2005 |
| Notes: | Fasting/refed 8 week old male C57BL6/J mouse was fasted for 20 hr to induce glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group (refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen RNeasy kit. |
| Tags: | Cancer, CREB Targets, ES Cell, Gene Atlas |
| Correlation Tags: | |