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About This Project

Project:Mouse Fasting Liver and Hepatocyte w/ FSK
Species:Mouse
Date:2013-05-21
URL:PNAS 2005
Notes:Fasting/refed 8 week old male C57BL6/J mouse was fasted for 20 hr to induce glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group (refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen RNeasy kit.
Tags:CREB Targets
Correlation Tags:

About The Data

Platform:Mouse Genome 430 2.0
Import Status:Verified, Ready To Use
Import Status Details:Good
No.Original NameDisplay NameTypeLog ScaleStatistical Value
Categories
ExperimentsCondition #1
1.probe setprobe setIdentifierIdentifierIdentifier
2.FastingFastingDataLinearLinearFastingFasting
3.RefedRefedDataLinearLinearFastingRefed
4.Hepatocyte 0hrHepatocyte 0hrDataLinearLinearFSK Time Course0hr
5.Hepatocyte 1hr FSKHepatocyte 1hr FSKDataLinearLinearFSK Time Course1hr
6.Hepatocyte 4hr FSKHepatocyte 4hr FSKDataLinearLinearFSK Time Course4hr
7.(Fasting vs Refed) FC(Fasting vs Refed) FCStatistical ValuesLinearFCFasting
8.(Fasting vs Refed) LFC(Fasting vs Refed) LFCStatistical ValuesLinearLFCFasting
9.(Hepatocyte 1hr FSK/0hr) FC(Hepatocyte 1hr FSK/0hr) FCStatistical ValuesLinearFCFSK Time Course1hr
10.(Hepatocyte 1hr FSK/0hr) LFC(Hepatocyte 1hr FSK/0hr) LFCStatistical ValuesLinearLFCFSK Time Course1hr
11.(Hepatocyte 4hr FSK/0hr) FC(Hepatocyte 4hr FSK/0hr) FCStatistical ValuesLinearFCFSK Time Course4hr
12.(Hepatocyte 4hr FSK/0hr) LFC(Hepatocyte 4hr FSK/0hr) LFCStatistical ValuesLinearLFCFSK Time Course4hr

» View Details

Platform:Mouse Genome 430 2.0
Type:Expression Array
Manufacturer:Affymetrix
Species:Mouse
Build Version:mm9
Release Date:2011-06-09
URL:URL
Description:The GeneChip® Mouse Genome 430 2.0 Array offers complete coverage of the Mouse Expression Set
430 for analysis of over 39,000 transcripts on a single array.
Notes:
Annotation File Status:Verified, Ready To Use
Import Status:Good
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